Weak Acid Hydrolysis of Proteins

Hydrolysis of Proteins with p-Toluenesulfonic Acid

A new procedure for analyses for tryptophan in proteins and glycoproteins is described. The method utilizes ptoluenesulfonic acid as the catalyst for hydrolysis and the amino acid analyzer for the quantitative estimation of tryptophan. The hydrolysis of proteins is carried out in 3 N p-toluenesulfonic acid containing 0.2 % 3-(Z-aminoethyl)indole in evacuated sealed tubes at 110” for 22, 48, and...

The liberation of aspartic acid during the acid hydrolysis of proteins.

Acid Hydrolysis of Pretreated Palm Oil Lignocellulosic Wastes

Palm oil solid wastes consist of cellulose, hemicellulose and lignin. In this study, a single stage of acid hydrolysis process of palm oil empty fruit bunch (EFB) for production of fermentable sugar was carried out under moderate temperature (45°C) and ambient pressure. The effect of four different process variables such as solid size, HCl concentration, solid percentage and temperature were in...

Hydrolysis of Sorghum (Broomcorn) in Diluted Hydrochloric Acid

Effective conversion of lignocellulosic material as renewable energy source has significant reflection on economic and environmental impact. Diluted acid hydrolysis at optimal condition was used to liberate fermentable sugar. The sorghum stalks, the alkali pretreatment of biomass and hydrolysis in diluted hydrochloric acid were investigated. The hydrolysis reaction was carried out in a 5L react...

Dilute Acid Hydrolysis of Papain

Studies on the amino acid sequence of papain by means of the that hydrolysis of endopeptide linkages in dilute acid might peptides from the chymotryptic (l-3) and tryptic digests (4, 5) proceed predominantly by a mechanism, independent of hydrogen of the protein have yielded a considerable number of partial ions, involving un-ionized P-carboxyl groups of aspartyl residue. sequences. Four of the...